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• DNA Analysis Considerations
  • Objectives
  • Substrates
  • Inhibitors
  • Overcoming Inhibition
• Organic Extraction
  • Objectives
  • Organic Extraction Process
    • Solubilization of Stain Components
    • Denaturation and Hydrolysis of Proteins
    • Removal of Denatured Proteins
    • DNA Purification
  • Techniques
  • Differential Method
• Chelex 100 Extraction
  • Objectives
  • Chelex^® 100 Extraction Process
    • Advantages and Disadvantages
    • Non-differential
    • Differential
• Other Extraction Techniques
  • Objectives
  • Modifications
    • Microcentrifuge Tubes with Spin Baskets
    • Centrifugal Filter Units
  • Other Techniques
    • Spin Column
    • Silica Beads
    • Magnetic Beads
    • FTA^® Technology
• Quantitation
  • Objectives
  • Overview
  • Yield Gels
  • Absorption Spectrophotometry
  • Fluorometry
  • Slot Blot Hybridization
  • AluQuant
  • Quantitative PCR
    • SYBR
    • Fluorescent Resonance Energy Transfer (FRET)
  • Data Analysis for Quantitative PCR
  • Summary of Advantages and Disadvantages